Development of a system to sensitively and specifically visualize c-fos mRNA in living cells using bispyrene-modified RNA probes.
نویسندگان
چکیده
In situ visualization of c-fos mRNA was shown both in fixed cells and in living cells using bispyrene-modified 2'-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fos mRNA was successfully developed.
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ورودعنوان ژورنال:
- Chemical communications
دوره 47 14 شماره
صفحات -
تاریخ انتشار 2011